Table 1 Proliferation dynamics of bovine AT-MSC per group. Data is reported as the mean ± standard deviation. PDT rows exclude values that exceed 3 days. Days to reach a number of PD were interpolated from proliferation data of each biological replicate via a linear function. N = 7 unless otherwise stated

PDT, days

P3

0.81 ± 0.12

1.22 ± 0.32

1.19 ± 0.56

1.21 ± 0.22

0.86 ± 0.2

1.40 ± 0.56

P6

1.32 ± 0.32

1.66 ± 0.65

(n = 5)

1.72 ± 1.12

0.91 ± 0.12

1.09 ± 0.42

1.18 ± 0.12

P9

2.01 ± 1.03 (n = 3)

1.81 ± 0.65

(n = 2)

1.48 ± 0.39

(n = 4)

1.77 ± 0.58

1.33 ± 0.46 (n = 6)

1.74 ± 0.69

(n = 4)

P12

1.38

(n = 1)

1.45

(n = 1)

2.41 ± 0.83

(n = 3)

1.93 ± 0.69 (n = 4)

1.84 ± 0.17 (n = 6)

1.79 ± 2.02 (n = 3)

P15

NA

1.27

(n = 1)

1.18 ± 0.15

(n = 2)

1.57 ± 0.2 (n = 2)

2.33 ± 1.02 (n = 5)

1.14

(n = 1)

Days to reach

10 PD

15.3 ± 4.81

23.34 ± 5.44 (n = 5)

16.76 ± 5.17

10.42 ± 0.81

15.48 ± 1.56

16.98 ± 3.56

20 PD

26.48 ± 8.81 (n = 5)

31.44 ± 10.06

(n = 2)

23.46 ± 3.87 (n = 5)

20.06 ± 2.66

25.57 ± 4.44 (n = 6)

35.64 ± 10.0(n = 6)

30 PD

28.78

(n = 1)

35.14

(n = 1)

38.78 ± 11.73

(n = 3)

33.54 ± 4.49 (n = 4)

37.37 ± 5.72 (n = 6)

42.69 ± 7.02 (n = 3)

40 PD

41.57

(n = 1)

50.72

(n = 1)

51.56 ± 4.8

(n = 2)

46.81 ± 4.34 (n = 3)

56.79 ± 5.29 (n = 6)

56.9 ± 9.35 (n = 2)

Maximum number of PD (mean ± SD)

26.06 ± 8.02

20.92 ± 13.1

30.76 ± 8.82

33.25 ± 7.17

38.00 ± 6.16

29.73 ± 9.5

Maximum number of PD (median [Q1– Q3])

21.97

[19.7-26.78]

11.81

[9.8-19.41]

27.63

[20.54–35.28]

32.98

[26.56-40]

40.00

[39.34-40]

20.81

[20.26–37.3]

Number of donors reaching 30 doublings

1/7

1/7

3/7

4/7

6/7

3/7