Fig. 4

ApoVs uptake by HUVECs depends on dynamin-, clathrin-, and caveolin-mediated endocytosis. The effects of (A) incubation time and (B) temperature on the internalization of DiO-ApoVs in HUVECs treated with 20 µg/mL ApoVs. (C) Schematic diagram of the endocytosis pathway in HUVECs. (D) Percentages of HUVECS positive for DiO-ApoVs in the presence of increasing concentrations of dynasore (12.5, 25, 50, and 100 µM), (E) chlorpromazine (6.25, 12.5, 25, and 50 µM), (F) Pitstop 2 (12.5, 25, 50, and 100 µM), (G) NYST (10, 20, and 40 80 µM), (H) MβCD (0.1, 1, 10, and 100 µg/mL), (I) heparin (0.1, 1, 10, and 100 µg/mL) and (J) EIPA (12.5, 25, 50, and 100 µM). All the data are representative of three independent experiments and are presented as the means ± SEMs. (n = 3; *P < 0.05 vs. ApoVs; **P < 0.01 vs. ApoVs)