Fig. 3

Expression level of IL-4, IL-10, and IL-13 in MSCs-3IL. (A) Fluorescence microscopy image showing MSCs-3IL cells expressing green fluorescent protein (eGFP), scale bar: 100 μm. (B) Flow cytometric analysis demonstrating the infection efficiency of MSCs-3IL at different passages. (C) QRT-PCR analysis of IL-4, IL-10, and IL-13 gene expression in MSCs-3IL compared to control MSCs. Statistical analysis was performed using one-way ANOVA. ***P < 0.001 compared to the control group. n = 3 biological replicates. Error bars represent standard deviations (SDs). (D) Western blot analysis showing protein expression levels of IL-4, IL-10, and IL-13 in MSCs-3IL. (E) ELISA results measuring IL-4, IL-10, and IL-13 secretion levels in MSCs-3IL cell supernatants collected after 72 h of culture. n.d: below the detectable range of ELISA. ***P < 0.001 compared to the control group. n = 3 biological replicates. Error bars indicate standard deviations (SDs)