Fig. 4

MSCs-3IL suppressed the inflammatory reaction and enhanced M2-like polarization in LPS-stimulated macrophages. (A) QRT-PCR analysis showing the expression levels of IL-10, IL-13, Arg1, IL-1β, IL-6, and TNFα in LPS-stimulated macrophages treated with MSCs-3IL compared to control conditions. Statistical analysis was performed using one-way ANOVA.*P < 0.05, **P < 0.01, ***P < 0.001. n = 3 biological replicates. Error bars represent standard deviations (SDs). (B) Flow cytometric analysis depicting the percentage of CD163-positive (M2 marker) and CD80-positive (M1 marker) cells in LPS-stimulated macrophages treated with MSCs-3IL