Fig. 8

NPS-EMSCs promote NSC proliferation and neuronal differentiation by activating the PI3K/AKT/GSK3β signaling pathway. (a) EdU staining of MK2206-pretreated NSCs co-cultured with NPS-EMSCs. Scale bar = 50 μm. (b) Immunofluorescence staining of MAP2 (green) and GFAP (red) in MK2206-pretreated NSCs co-cultured with NPS-EMSCs; scale bar = 50 μm. (c) Western blot analysis of PI3K, AKT, and GSK3β phosphorylation levels in rats with SCI(n = 6). (d) Statistical analysis of EdU-positive cells. (e) Statistics for MAP2 (green)-positive cell. (f-h) Statistical analysis of PI3K, AKT, and GSK3β phosphorylation levels. * P < 0.05, **P < 0.01, ***P < 0.001, ns: no significant difference