Fig. 10

Transplanted photoreceptor sheets in both Nude TP and NN TP IS groups demonstrate similar engraftment within host retina architecture. a, d Transplant to nude RD rat (Nude TP). b, e Transplant to non-nude immunosuppressed RD rat (NN TP IS). c, f Normal rat retina (WT). a–c Calretinin positive (green) retinal ganglion cells and protein kinase C α (PKCα) positive (red) rod bipolar interneurons survive in both immunocompromised (a) and immune competent (b) retinal degenerate rats alike. However, in RD, these layers present with increasing levels of disorganization and destratification compared to non-RD wild-type (c). There is few Calretinin but a lot of PKCα staining in the transplant (a, b). d, e CSC14-derived RO transplants (labeled by human nuclear marker Ku80, green) contain mature rod photoreceptors expressing rhodopsin [40] which are organized in rosettes with outer segments in center, in contrast to the layered WT retina (f). Scale bars = 100 µm for the overviews, 50 and 20 µm for the enlargements