Fig. 5

Immune phenotyping. a Representative flow plots of standard immune phenotyping gate scheme and its application to phenotyping of non-surgery untreated immunocompetent control (NN AMC) and IS treated (NN TP IS), immune competent transplanted RD rats in comparison to transplanted immunodeficient (nude/foxn1−/−) rat (Nude TP). b Key lymphocyte populations in non-nude TP IS group over treatment course. With dosing regimens #5, 20 mg TAC+ 150 mg MMF and #6, 20 mg TAC and 300 mg/kg, respectively. Treatment resulted in substantial suppression of CD3+ T-cell populations, expansion of CD3−CD8+ natural killer lymphocytes. B-cells undergo suppression in the initial 0–30-day time interval of treatment, followed by subsequent trend of expansion thereafter. Statistical analyses were performed using ordinary one-way ANOVA with Dunnet multiple comparisons. The statistical significance of each lymphocyte subpopulation census at each treatment timepoint is obtained by comparison to representative mean lymphocyte subpopulations from immunocompetent non-treatment, non-surgery age matched littermates