Table 2 Overview of immunosuppression pilot experiments
From: Effect of immunosuppression on hESC-derived retina organoids in vitro and in vivo
Rat type | Treatment | Age at surgery | Days post-surgery (dps) | Age at death | Died on surgery day | Died pre-maturely | Cornea issues | Surgical trauma | Outcome |
|---|---|---|---|---|---|---|---|---|---|
Non-immunosuppressed rats (NN NO IS) = 18 rats (16M, 2 F)—pilot experiments | |||||||||
 Transplant (1F, 11M) | None | 37–42 d 38.1 ± 0.7) | 0–77 d (70.9 ± 8.5) | 36–114 d (70.9 ± 8.3) | 2/12 (17%) | 2/12 (17%) | 1/12 (8.5%) | 2/12 (17%) | TP rejection (43–77 dps) blood collection; histology |
 Sham (4M, 1F) | 37 d (37.0 ± 0) | 51–188 d (142.8 ± 25) | 88–225 d (179.8 ± 25) | 0 | 0 | 2/5 (40%) | 0 | blood collection; histology | |
 AMC (1M) | n.a | n.a | 104 d | n.a | n.a | n.a | n.a | blood collection; histology | |
IS rats TAC only (NN IS TAC) = 15 rats (10F, 5 M)—Pilot experiments | |||||||||
 Transplant (8F, 1M) | 10–20 mg 90-d TAC | 35–38 d (35.8 ± 0.3) | 0–82 d (33.4 d ± 9.8) | 36–117 d (69.2 d ± 9.8) | 2/9 (22%) | 4/9 (44%) | 2/9 (22%) | 1/9 (11%) | TP rejection: 2/9 (22%) |
 Sham (2M) | 35–37 d (36 d ± 1.0) | 0–69 d (34.5 ± 34.5) | 36–104 d (70.0 ± 34.0) | 1/2 (50%) | 1/2 (50%) | 0 | 0 | Blood collection, histology | |
IS rats MMF only = 12 rats (6M, 6F)—Pilot experiments | |||||||||
 Transplant (4F, 5M) | 100–300 mg/kg MMF in food | 35–44 d (39.7 ± 2.9) | 7–41 d (27.7 ± 2.9) | 51–85 d (67.6 ± 3.1) | 0 | 0 | 2/9 (22%) | 2/9 (22%) | TP shrinking in OCT; TP rejection 1/9 (11%); |
 Sham (2F) | 44 d (av. 44) | 41 d (av. 41) | 74–85 d (79.5 ± 5.5) | 0 | 0 | 0 | 0 | Blood collection; histology | |
 AMC (2M) | n.a | n.a | 66–233 d (149.5 ± 83.5) | n.a | 0 | 0 | n.a | Blood collection, histology | |