Fig. 2
Characteristics of the ME7 prion disease model. A Representative western blot analysis of total prion protein (PrP) and the abnormal isoform of PrP (PrPSc) in brain homogenates from ME7-infected and negative control mice (CTL, n = 3 per group) at 5 months post-injection. PrP-PK, no proteinase K treatment; PrP + PK, proteinase K treatment. B GFAP immunoblots analyzed by Western blotting (n = 3 per group). C Quantitative analyses of GFAP immunoblots from the experiments panel. ***p < 0.001. HSP90 served as the loading control. All data are expressed as the means ± SD. The student’s t-test was used to compare two groups. The full-length blot is presented in supplementary Figs. 1 and 2