Fig. 3

Properties of adipose-derived mesenchymal stem cells (AdMSCs) isolated from ME7-infected mice compared with the negative control. A Representative images of morphology (from one of four independent experiments) show changes in cell size due to ME7 infection (upper panel, low magnification and lower panel, high magnification). B Measurement of cell size shows a significant increase in the diameter of AdMSCs isolated from ME7-infected mice compared with the negative control group (n = 5). * p < 0.05. All data are expressed as the means ± SD. The student’s t-test was used to compare two groups. C Viability of AdMSCs from ME7-infected and negative control mice. * p < 0.05, ** p < 0.01. All data are expressed as the means ± SD. The student’s t-test was used to compare two groups. D Characterization of AdMSCs with flow cytometry through an immunophenotype analysis of MSC cell surface markers (CD105, CD90, and CD44) and a hematopoietic marker (CD34) (n = 3). E Percentage means of MSC markers from the two groups (n = 3). F Mean fluorescence intensity (MFI) of MSC markers from the two groups (n = 3). All data are expressed as the means ± SD. The student’s t-test was used to compare two groups