Fig. 2

CD73 was knockout in the ERC derived exosome. A Flow cytometry reflected CD73 expression on the membrane of ERC and CD73^−/−ERC. B Electron microscopy images of exosome from ERC-Exo and CD73^−/−ERC-Exo (Scale bar: 200 nm). C Nanoparticle tracking analysis (NTA) of exosome from ERC-Exo and CD73^−/−ERC-Exo. D Protein biomarkers of ERC-Exo and CD73^−/−ERC-Exo (CD9, CD63, CD81, and Calnexin) were performed. Full-length blots/gels are presented in Additional file 1: Fig. S1. E Expression of CD73 in ERC-Exo and CD73^−/−ERC-Exo via Western blotting. Full-length blots/gels are presented in Additional file 1: Fig. S2. F Gray value analysis with immunoblot based; CD73 intensity analysis was homogenized after comparing to CD9 (n = 3). G, H Levels of inorganic phosphate (Pi) and Adenosine were measured after adding CD73 substrate (n = 3). Statistical analysis was done by using unpaired two-tailed Student’s t-tests. Data are presented as mean ± s.e.m (SEM). ****P < 0.0001, analyzed by unpaired t-test. ERC, endometrial regenerative cell; CD73^−/−ERC, ERC transfected with lentivirus; Exo, exosome