Fig. 2

S/MAR DNA vector induced EPO overexpression. A. Schematic overview of the generation of EPO and GFP overexpressing iPSC using S/MAR DNA vectors. B. GFP expression in iPSC over a 67-day expansion period and 10 passages (scale bar = 200 μm). C. EPO release rate by control and EPO + iPSC. Data are presented as mean ± SD. Unpaired t-test with Welch’s correction was used to test for significance, n = 4. D. GFP expression in kidney organoid at day 21 of differentiation. E. Relative EPO mRNA expression in control and EPO + kidney organoids. Data are shown as median (IQR). Welch’s t-test was used; n = 15 for control, n = 13 for EPO+. F. EPO release in culture medium by control and EPO + kidney organoids. Data are shown as median (IQR). Welch’s t-test was used; n = 6. *: p < 0.05, **: p < 0.01, ***: p < 0.001