Table 3 Immunohistochemistry information
From: Erythropoietin delivery through kidney organoids engineered with an episomal DNA vector
Antibody | Dilution | Species | Company | Clone | Pretreatment in minutes | Ab incubation time at 37ËšC |
|---|---|---|---|---|---|---|
PDGFRb | 1/100 | Rabbit | Invitrogen | Polyclonal | CC1 32’ OV | 32 min |
PODXL | 1/3000 | Rabbit | Abcam | EPR9518 | CC1 32’ OV | 32 min |
WT1 | 1.24 µg/ml | Mouse | Cell Marque | 6 F-H2 | CC1 64’ UV | 32 min |
Villin | 1/600 | Rabbit | Abcam | Polyclonal | CC1 32’ OV | 32 min |
ECAD | 3.54 µg/ml | Mouse | Ventana | 36 | CC1 32’ OV | 20 min |
CD31/PECAM1 | 6.36 µg/ml | Mouse | Ventana | JC70 | CC1 32’ OV | 48 min |
Human CD31/PECAM1 (ab9498) | 0.5 µg/ml | Mouse | Abcam | JC/70A | CC1 32’ OV |  32 min |