Fig. 1

Cellular distribution and composition. A–D Representative images showing the cell distribution following cell seeding stained for DAPI in blue. Scale bar at 75 µm. E Cell density quantification at the border and center of the constructs, for static and perfusion conditions, respectively. Measurements were done following cell seeding for n = 4 samples from 2 independent experiments. F Relative cellular compositions of three SVF donors pooled were analyzed by flow cytometry of the SVF at day 0, after cell seeding yet before in vivo implantation for static and perfusion conditions respectively. Pericytes: CD45⁻, CD146⁺, CD34⁻; MSC: CD45⁻, CD73⁺, CD90⁺; progenitor EC (Endothelial cells): CD45⁻, CD34⁺, CD31⁺; Mature EC: CD45⁻, VEGFR2⁺, CD31⁺; Myeloid cells: CD45⁺, VEGFR2⁺, CD11b⁺. All statistical analyses were performed using an unpaired student's t-test. (* p < 0.05, *** p < 0.001)