Fig. 5

In vivo patch vascularization (A) Representative immunofluorescent images stained for DAPI (blue) and CD31 (red). Scale bar at 250 µm. (B) Quantitative analysis of lectin positive vessel-like structures at 28 days. (C) VLD analysis between the border and center of constructs. At 28 days no distinction is made for the border or center and analysis was performed on the whole construct. Statistical analysis was performed using a student's t-test and a one-way ANOVA (** p < 0.01, *** p < 0.001, **** p < 0.0001). Analysis for VLD was performed on n = 4 samples at 3 days, 3 samples at 7 days and 6 samples at 28 days. Analysis for % of lectin-positive vessels was performed on 5 samples at 28 days