Fig. 4

The three directions of AdMSC differentiation are shown. A) Intracellular staining of fat vacuoles with Oil Red O. B) Histological section through a 3D chondro-pellet after chondrogenic differentiation. The sulfated proteoglycans were stained with Alcian blue. C) Distinct nodule formation after osteogenic differentiation with calcium accumulation clearly stained red by Alizarin staining. The scale bar corresponds to 100 μm