Fig. 5

Assembly into 3D spheroids and application of poly(I:C) synergistically increase the survival of MSCs following intraperitoneal transplantation into mice with IBD. (a) Representative phase-contrast images of MSCs subjected to various treatments followed by exposure to oxidative stress and (b) corresponding results from the cell counting kit-8 (CCK-8) assay (n = 4). Scale bar, 300 μm. (c) Representative in vivo bioluminescence images of mice receiving MSC transplantation and (d) the corresponding bioluminescence intensities (n = 4 animals per group). The data are represented as the means ± SDs. ANOVA followed by Tukey’s test was used to determine the p values. *p < 0.05; **p < 0.01; ****p < 0.001; ns, not significant