Fig. 1

IMRCs inhibit fibrosis in TGF-β1-induced HESCs ex vivo. (a) Schematic diagram for the group of IUA cell experiment procedure. HESCs group, HESCs + TGF-β1group, HESCs + TGF-β1 IMRCs-CM group, and HESCs + IMRCs-CM group. (b) Representative morphology of HESCs, with or without the conditioned media of IMRCs, along with TGF-β1 treatment. Scale bar, 100 μm. (c) RT-qPCR for the relative expression of α-SMA, Vimentin, N-Cadherin, and E-Cadherin in different experimental groups. (d) Western blot for the relative expression of α-SMA, Vimentin, N-Cadherin, and E-Cadherin in different experimental groups. (e) Statistical analysis of Western blot. ∗p < 0.05, ∗∗∗p < 0.01, ∗∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; data are represented as the mean ± SD