Fig. 2

Localization, distribution and residence of IMRCs in IUA rats. (a) Localization of of IMRCs in the rat endometrium. Immunofluorescence images of the localization of DiR-labeled IMRCs (red) in the endometrium at day 3, 7, 14, and 21 after treatment. Scale bar: 100 μm. (b) Residence and distribution of IMRCs in IUA rat. In vivo bioimaging imaging at day 3, 7, 14, and 21 after intrauterine injection of IMRCs labeled with DIR. Ex vivo imaging of major organs at day 3, 7, 14, and 21 after uterine infusion of IMRCs labeled with DiR. The isolated organs in order from top to bottom are heart, lungs, liver, spleen, kidneys, ovaries and uteri. (c) Schematic diagram for the construction and treatment of the IUA rat. (d) Surgical procedures for the construction and treatment of the IUA rat model