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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Human embryonic stem cell-derived immunity-and-matrix-regulatory cells promote endometrial repair and fertility restoration in IUA rats

Fig. 7

Exos participate in the regulation of the TGF-β/Smad signaling pathway. (a) Western blot analysis of Smad2 and Smad3 phosphorylation levels in cells following induction. (b) Statistical analysis of Western blot. (c) KEGG pathway analysis of the top 30 miRNAs. (d) miRNA-Gene network map. (e) RT-qPCR for the relative expression of miR-27b-3p, miR-145-5p, and miR-16-5p. (f) Western blot detection of fibronectin expression (FN, COL1A1and α-SMA). (g) Statisticalanalysis of Western blot (FN, COL1A1and α-SMA). (h) CCK8 assay to assess HESCs proliferation. （i-j） Scratch assay to evaluate cell migration.

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Stem Cell Research & Therapy

ISSN: 1757-6512