Fig. 1

Construction of DSUP modified hMSCs. A Schematic diagram of DSUP lentiviral vector backbone; B GFP ratio of lentiviral transfected hMSCs before and after sorting by flow cytometer, bar = 25 μm; C GFP immunofluorescence of hMSCs after sorting and purification; D Detection of DSUP target gene fragments in each group of hMSCs (1200–1500 bp); E qRT-PCR to detect the expression of DSUP mRNA in each group of hMSCs. The mRNA expression level of wildtype MSCs was normalized to 1, n = 3, Mean ± SEM, **p < 0.01, by Student’s t-test. F–G Immunofluorescence detection of DSUP-Flag fusion protein expression in each group of MSCs, DSUP-Flag was fluorescently labeled in red, and GFP protein was fluorescently labeled in green, with bar = 100 μm. Statistical analysis of the proportion of DSUP-Flag expression in each group of MSCs was plotted, n = 3, Mean ± SEM, ***p < 0.001, by Student’s t-test