Fig. 3
Validation of the anti-apoptotic and DNA protection ability of DSUP-modified MSCs against ROS and radiation damage. A Cell morphology images of MSCs in each group 24 h after 15 mmol H2O2 treatment. Scale bar is 200 μm; B Flow cytometry detection of apoptosis and statistical histograms of MSCs in each group in Figure A. Early apoptosis was labeled with Annexin V, and late apoptosis was labeled with 7-AAD, n = 3, Mean ± SEM, **p < 0.01, ***p < 0.001, by Student’s t-test; (C) Comet assay images and statistical plots of MSCs in each group after treatment with 12 Gy X-ray radiation, n = 50, Mean ± SEM, ***p < 0.001, by Student’s t-test. Scale bar is 100 μm; D Immunofluorescence images of γH2AX in MSCs of each group after 12–24 h treatment with 12 Gy X-ray radiation, scale bar is 50 μm. Statistical analysis of γ-H2AX foci expression rate plotted by mean fluorescence intensity of cell nuclei reflecting γ-H2AX foci expression rate, n = 3, Mean ± SEM, **p < 0.01, ***p < 0.001, by Student’s t-test