Fig. 5

Comparative analysis of HSPCs-related properties of radiation co-culture models in each group. A Morphological maps and Giemsa staining images of each group HSPCs in co-culture model after 9 days of radiation, NC group is the same batch of unirradiated mHSPC cells, bright field image scale = 100 μm, Giemsa image scale = 20 μm; B Flow cytometry t-SNE downscaling plots of LSK ratios of each group HSPCs in Figure A. Lin⁻ cells are shown in blue and LSK cells are shown in red; C Statistical analysis plot of the LSK ratios in each group of HSPCs, n = 3, Mean ± SEM, *p < 0.05, **p < 0.01, by Student’s t-test; D Statistical plot of the hematopoietic colonies counts of each group HSPCs in MSCs-HSPCs radiation co-culture model, n = 4, Mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, by Student’s t-test; E Typical images of hematopoietic colony formation of HSPCs in the radiation co-culture model for each group, scale bar = 500 μm; F Statistical plots of the different lineages hematopoietic colonies counts of HSPCs in each group of Figure E, n = 4, Mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, by Student’s t-test; G Statistical plot of the total cell counts from hematopoietic colonies of HSPCs in each group of Figure E, n = 4, Mean ± SEM, **p < 0.01, ***p < 0.001, by Student’s t-test