Fig. 7

DSUP modified MSCs promoted the recovery of residual HSPCs in irradiated mice. A Typical flow cytometry plots of human MSCs in the mCD45⁻ cells subpopulations of BM karyocyte cells in two groups irradiated mice. B Representative fluorescent images of human MSCs detected in the Fig. 7A, scale bar = 10 μm. C Statistical plots of tibias BM mononuclear cells count in three groups mice, Mean ± SEM, ***p < 0.01, by Student’s t-test. D Typical flow cytometry t-SNE downscaling plots of different stages of hematopoietic cells proportion in each group mice, HSPCs: Lin⁻c-Kit⁺CD150⁺ cells, Lin⁻ progenitors: Lin⁻ cells, c-Kit⁺ progenitors: c-Kit⁺ cells, mature cells: Lin⁺c-Kit⁻ cells. (E) Statistical plots of the proportion of HSPCs, Lin⁻ progenitors, c-Kit⁺ progenitors and mature hematopoietic cells in each group mice detected in Fig. 7 D, Mean ± SEM, *p < 0.05, **p < 0.01, by Student’s t-test