Fig. 3

The PKM2 tetramer likely mediates glycolysis in BMSCs under HG inflammatory microenvironment. (A, B) The relative expression of glycolysis-related genes and proteins (LDHA, PFKFB3, and PKM2) was assessed by qPCR and western blot analysis on day 7. Full-length blots are presented in Supplementary Fig. S3. (C) Quantification of relative protein expression levels of the PKM2 tetramer, PKM2 dimer, and the tetramer/dimer ratio in BMSCs, presented as bar graphs. Full-length blots are presented in Supplementary Fig. S3 (D) Representative fluorescence images and 3D surface plots showing PKM2 and nuclei in BMSCs (Nucleus: blue, PKM2: red; scale bar: 100 μm). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; ns, not significant