Fig. 8

Shikonin reduces alveolar bone loss in diabetic periodontitis mice and promotes periodontal tissue remodeling. (A) Schematic diagram of modeling process of diabetes periodontitis mice (Images created with Biorender.com). (B) Micro-CT images of alveolar bone in each group, including 3D and sagittal views. Red areas indicate the extent of exposed tooth roots. (C) Analysis of the CEJ-ABC distance and the bone morphological parameters of the alveolar bone in each group. (D-F) H&E, Masson, and TRAP staining of periodontal tissue. (scale bar: 100 μm). (G) Representative IHC staining images of RUNX2 and PKM2 in periodontal tissue. (scale bar: 100 μm). (H) IF staining of OCN and PKM2 in the periodontal region. (Nucleus: blue, PKM2: red, OCN: green; scale bar: 100 μm). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; ns, not significant