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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Poly-ε-caprolactone/chitosan/whitlockite electrospun bionic membrane conjugated with an E7 peptide for bone regeneration

Fig. 4

To evaluate the effect of different membranes on osteogenic differentiation of BMSCs in vitro. (A) Sirius staining was used to observe the collagen content of BMSC cultured with the extract of each nanofiber membrane was performed after 7 and 10 days of osteogenic induction. (B) The semi-quantitative analysis of Sirius staining. (C) After 10 days of osteogenic induction, alizarin red staining was used to observe the calcium nodule content in BMSCs cultured with different membranes extracts. (D) The semi-quantitative analysis of Alizarin red staining. (E-H) The RT-qPCR results for osteogenic genes ALP, BMP-2, OCN and RUNX2 were obtained from the nanofibrous membranes. ( n = 3 *p < 0.05, **p < 0.01)

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Stem Cell Research & Therapy

ISSN: 1757-6512