Fig. 7

Inhibition of glutamine metabolism and KLF6 attenuated tertiary dentin formation. (A) Representative µCT images of maxillary first molars from mice received intraperitoneal injections of BPTES or vehicle control. (B) Representative HE and IHC staining images of DSPP and DMP1. (C) The ratio of coronal pulp based on µCT images (control group: n = 11, BPTES group: n = 10). (D) The ratios of DSPP and DMP1 positive staining areas based on IHC staining images (control group: n = 5, BPTES group: n = 6). (E) Representative µCT images of maxillary first molars from mice received injection of AAV6-shKLF6 or AAV6-shNC. (F) Representative HE and IHC staining images of DSPP and DMP1. (G) The thickness of dental bridges based on HE staining images (n = 10). (H) The ratios of DSPP and DMP1 positive staining areas based on IHC staining images (n = 6). Data were analyzed using Student’s t-test in (C–D, and G–H). * P < 0.05, ** P < 0.01, and *** P < 0.001