Table 4 Summary of clinical studies on the effect of conditioned medium of mesenchymal stem cells on wound healing

LI G, 2024 [24]

Assessment of the advantages of adipose-derived stem cell-conditioned medium (ADSC-CM) in promoting wound healing following fractional CO₂ laser treatment on human skin.

Nineteen patients aged 24 to 33 underwent treatment on both inner arms using a fractional CO₂ laser. Each side was irradiated at energy levels of 8 and 16 millijoules with identical spot density set at 30% (four treatment sites total, each measuring 1 cm²). Following laser treatment, ADSC-CM (adipose-derived stem cell-conditioned medium) was applied topically to a randomly chosen arm for one hour, while DMEM was applied to the other arm for comparison.

Assessment of outcomes was conducted on days 1, 4, 7, 14, and 21 post-laser treatment using a TEWAmeter, Mexameter, and Cutometer.

The ADSC-CM-treated skin showed a significant reduction in erythema, melanin, and TEWL index compared to the control group. Additionally, type III procollagen mRNA expression was 2.6 times higher in the ADSC-CM-treated group than in the control group. The application of ADSC-CM allografts demonstrated notable effectiveness in minimizing transient side effects, such as erythema and hyperpigmentation, while improving TEWL to enhance wound healing following fractional CO₂ laser treatment.

Pourhashemi, 2024 [37]

To evaluate the combined and individual effects of photobiomodulation (PBM) and conditioned medium derived from human adipose tissue-derived stem cells (h-ASC-CM) on ischemic infected delayed healing wounds (IIDHWM) in rats with type I diabetes mellitus (TIDM).

Four groups were assessed: control (group 1), h-ASC-CM (group 2), PBM (group 3), and combined treatment (group 4).

Evaluation of wound healing response in each group.

The study demonstrated the impact of PBM, h-ASC-CM, and their combination on improving wound healing outcomes in rats with TIDM.