Fig. 1

Schematics of experimental design. (A) Human iPSC seeding conditions; (B) Differentiation timeline; (C) Cell characterizations; and (D) Extracellular vesicle characterizations. Ag: aggregates grown in the VWBR. MC: cells attached to Matrigel coated microcarriers in the VWBR. 6-well: aggregates grown as static culture in 6-well plates. 40: 40Â rpm; 80: 80Â rpm. Created by BioRender