Fig. 3
From: Generation and purification of iPSC-derived cardiomyocytes for clinical applications
Human iPSC-derived cardiomyocytes show a cardiac phenotype. (A) Schematic representation of the timeline and experimental setup. (B) After differentiation of iPSC into cardiomyocytes immunostaining was performed for following markers: alpha smooth muscle actin (αSMA), cTNT, Myl2, Oct4, Ki67, Nanog and Dapi. (C) Flow cytometry dot plot of Oct4, cTnT and SIRPA expression for iCMs DW4 and undifferentiated iPSCs. (D) Quantitative RT-PCR assay for expression analyses of mesodermal, cardiac and pluripotent markers at day10 and day17 after differentiation. Data were normalized with GAPDH and relative to the undifferentiated iPSCs. Quantitative data are presented as mean ± standard deviation. One representative clone DW4 is shown. MIM: Mesoderm Induction Media, CMM: Cardiac Maintenance Media, CIM: Cardiac Induction Media, CIM II: Cardiac Induction Media II. (Scale bars: 100 μm)