Fig. 5
From: Generation and purification of iPSC-derived cardiomyocytes for clinical applications
Human iPSC-derived cardiomyocytes can be replated and frozen/thawed for later applications without phenotype change. (A) Quantitative RT-PCR assay for expression analyses of mesodermal, cardiac and pluripotent markers at day10 after differentiation, after replating (P1) and after freeze/thawing (F/T). Data were normalized with GAPDH and relative to the undifferentiated iPSCs. Quantitative data are presented as mean ± standard deviation. (B + C) Immunostaining for different markers (alpha smooth muscle actin (αSMA), Nkx2.5, cTNT, Myl2, Nanog, Ki67, Actinin, Oct4, and Dapi) of cultured iCMs after replating or freeze/thawing. (D) Pie charts of analyzed flow cytometry show percentage of iCM (red, cTNT positive) and iPSC (blue, Oct4 positive) for the respective marker after replating or freeze/thawing. One representative clone DW4 is shown