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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Sildenafil promotes osteogenic differentiation of human mesenchymal stem cells and inhibits bone loss by affecting the TGF-β signaling pathway

Fig. 3

Sildenafil at 10 mg/L promoted osteogenic differentiation of hBMSCs in the subcutaneous ectopic osteogenesis model. A. H&E staining of the PM and the PM + sildenafil groups; B. Masson trichrome staining of the PM and the PM + sildenafil groups; C. Immunohistochemical staining for OCN of the PM and the PM + sildenafil groups; D. Statistical analysis of the heterotopic bone area/tissue area (%) in the H&E staining of the PM and PM + sildenafil groups; E. Statistical analysis of the collagen area/tissue area (%) in the Masson staining of the PM and PM + sildenafil groups; F. Statistical analysis of the relative IOD in the immunohistochemical staining for OCN of the PM and PM + sildenafil groups. [scale bars]: 500 μm (10x) and 100 μm (40x). hBMSCs, human bone marrow-derived mesenchymal stem cells; H&E, hematoxylin and eosin; PM, proliferation medium; OCN, osteocalcin

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Stem Cell Research & Therapy

ISSN: 1757-6512