Stem Cell Research & Therapy

Table 1 The primer sequences

From: Sildenafil promotes osteogenic differentiation of human mesenchymal stem cells and inhibits bone loss by affecting the TGF-β signaling pathway

Genes	Forward (5ʹ to 3ʹ)	Reverse (5ʹ to 3ʹ)
GAPDH	CGGACCAATACGACCAAATCCG	AGCCACATCGCTCAGACACC
ALP	GACCTCCTCGGAAGACACTC	TGAAGGGCTTCTTGTCTGTG
RUNX2	TCTTAGAACAAATTCTGCCCTTT	TGCTTTGGTCTTGAAATCACA
BGLAP	AGCAAAGGTGCAGCCTTTGT	GCGCCTGGGTCTCTTCACT
TGF-β1	CAATTCCTGGCGATACCTCAG	GCACAACTCCGGTGACATCAA
TGF-βR1	TCAGCTCTGGTTGGTGTCAG	ATGTGAAGATGGGCAAGACC
TGF-β2	TCAAGAGGGATCTAGGGTGGAA	GGCARGCTCCAGCACAGAA
TGF-βR2	AATATCCTCTGAAGAACGACCTAA	TCCCACCTGCCCACTGTTA

GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ALP, alkaline phosphatase; RUNX2, runt-related transcription factor 2; BGLAP, bone gamma-carboxyglutamate protein; TGF-β1, transforming growth factor-β1; TGF-βR1, transforming growth factor-β type I receptor; TGF-β2, transforming growth factor-β2; TGF-βR2, transforming growth factor-β type II receptor

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ISSN: 1757-6512

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