Fig. 1
From: Nano-zinc oxide (nZnO) targets the AMPK-ULK1 pathway to promote bone regeneration
nZnO promotes bone regeneration in mouse calvarial defects. (A) Schematic illustration of in vivo experiment design. (B) Micro-CT 3D reconstruction of craniums in the control and nZnO groups 4 and 8 weeks after surgery. Scale bars: 2 mm, Magnified Scale bars: 1 mm. (C) Quantitative analysis of micro-CT imaging of defect regions (n = 6 in each group). (D) Histological staining of craniums from the control and nZnO groups, including H&E and Safranin O-Solid Green staining. Scale bars: 1 mm, Magnified Scale bars: 500 μm. (E) Quantitative analysis of the new bone area within the defect tissue in D) histology images (n = 6 in each group). Data were presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 relative to control