Fig. 2

JPH2-knockout cardiomyocytes exhibit hypertrophy and heart failure-like phenotypes. (A) Flow cytometry analysis of cell size (forward scatter) in WT-CMs and KO-CMs on Day15, 22, and 30 post-differentiation (10,000 cells/sample). (B) Bar graph showing the increase in cell volume of KO-CMs over time. (C) Immunofluorescence staining of TNNT2 and α-actinin in WT-CMs and KO-CMs at day 30 post-differentiation: α-actinin in green, cTnT in red, DAPI in blue. Scale bar = 50 μm. (D) Quantification of disorganized sarcomeres in WT-CMs and KO-CMs. (E) qRT-PCR analysis of heart failure marker genes (TNNT2, TNNI3, MYH7/MYH6, ANP, BNP) in WT-CMs and KO-CMs at day 30 post-differentiation. Results are presented as means ± S.E.M. of 3 independent experiments. ns, not significant, **P < 0.01; ***P < 0.001; ****P < 0.0001, unpaired two-sided Student’s t test