Fig. 3

JPH2 knockout disrupts excitation-contraction coupling in cardiomyocytes. (A) Representative contractile waveforms of WT-CMs and KO-CMs recorded by the CardioExcyte96 system at day 30 post-differentiation. (B-E) Statistical analysis of contractile parameters (amplitude, Peak to Peak time, Time to Peak, Relaxation Time) in WT-CMs and KO-CMs. (F) Representative calcium transient images and waveforms of WT-CMs and KO-CMs captured by confocal microscopy at day 30 post-differentiation. (G-J) Statistical analysis of calcium transient parameters (amplitude, Peak to Peak Time, Time to Peak, Delay time) in WT-CMs and KO-CMs. (K) Representative TEM images of the JMC structure in WT-CMs and KO-CMs at day 30 post-differentiation. Scale bar = 100 nm. (L) Bar graph showing the distance between the PM and SR membrane in WT-CMs and KO-CMs; n = 6. Results are presented as means ± S.E.M. of 4 independent experiments. **P < 0.01; ***P < 0.001; ****P < 0.0001, unpaired two-sided Student’s t test. Abbreviations: WT, WT-CMs; KO: KO-CMs