Fig. 3
Assessment of cell viability in iNeurons cultures exposed to Aβ-42 oligomers (Aβ-42o), or Aβ-42o and extracellular vesicles from human induced pluripotent stem cell-derived neural stem cells (hiPSC-NSC-EVs). The bar chart in A shows the results of an MTT assay comparing the percentages of viable neurons in iNeurons cultures exposed to different concentrations of Aβ-42o. The bar charts B-C show the results of MTT assays comparing the percentages of viable neurons in iNeurons 1 and 2 cultures exposed to Aβ-42o or Aβ-42o and different concentrations of hiPSC-NSC-EVs. Figures (D-H) and (J-N) are representative images from cultures of iNeurons 1 (D-H) and iNeurons 2 (J-N), processed for live and dead cell assay. (D) and (J), control iNeurons 1 and 2 cultures. (E) and (K), iNeurons 1 and 2 cultures exposed to Aβ-42o. (F) and (L), iNeurons 1 and 2 cultures exposed to Aβ-42o and treated with 1.5 × 109 billion EVs. (G) and (M), iNeurons 1 and 2 cultures exposed to Aβ-42o and treated with 3.0 × 109 billion EVs. (H) and (N), iNeurons 1 and 2 cultures exposed to Aβ-42o and treated with 6.0 × 109 billion EVs. The bar charts (I) and (O) compare the area fractions of green fluorescence (implying dead cells) across different groups for iNeurons 1 (I) and iNeurons 2 (O) cultures. The experiments comprised measurements from 3 biological replicates/group. Scale bar, 100 μm