Fig. 4
The effects of adding extracellular vesicles from human induced pluripotent stem cell-derived neural stem cells (hiPSC-NSC-EVs) into iNeurons cultures on Aβ-42 oligomers (Aβ-42o)-induced neurodegeneration. Figures (A-E) and (H-L) show representative images of surviving microtubule-associated protein-2 (MAP-2) positive neurons from control cultures of iNeurons 1 and 2 (A, G), and iNeurons 1 and 2 cultures exposed to Aβ-42o alone (B, H), Aβ-42o and treated with 1.5 × 109 billion EVs (C, I), Aβ-42o and treated with 3.0 × 109 billion EVs (D, J), or Aβ-42o and treated with 6.0 × 109 billion EVs (E, K). Scale bar, 100 μm. The bar charts in F and L compare the numbers of surviving MAP-2 + neurons per unit area (0.35mm2) of iNeurons 1 and iNeurons 2 cultures across various groups. The experiments comprised measurements from 3 biological replicates/group