Fig. 7
The effects of adding extracellular vesicles from human induced pluripotent stem cell-derived neural stem cells (hiPSC-NSC-EVs) into iNeurons cultures on Aβ-42 oligomers (Aβ-42o)-induced impairment of mitochondrial membrane potential. Figures (A-I) are representative images of red and green fluorescence from a JC-1 assay in neuronal cultures from a control group (A, D, G), Aβ-42o treated group (B, E, H), or Aβ-42o and 6 × 109 hiPSC-NSC-EVs treated group (C, F, I). The bar charts compare the ratio of red/green fluorescence measured through fluorimetry (J) or area fraction (AF) analysis using Image J (K) across control, Aβ-42o exposed, and Aβ-42o and 6 × 109 hiPSC-NSC-EVs treated groups. Scale bar, 100 μm. The experiments comprised measurements from 3 biological replicates/group