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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Footprint-free induced pluripotent stem cells can be successfully differentiated into mesenchymal stromal cells in the feline model

Fig. 6

Successful generation of feline iPSC-derived mesenchymal stromal cells (MSCs). (A). Morphology of iPSC-derived MSCs-1 and − 2, with evenly distributed plastic adherent cells. (B). Conventional RT-PCR analysis of feline MSC-specific surface markers CD29, CD44, CD73, CD90, and CD105, the hematopoietic stem cell marker CD34, and the loading control GAPDH, in feline iPSC-MSCs and iPSCs. Full-length gels are presented in Additional File 10: Fig. S10. (C & D). Immunofluorescence (C) and flow cytometric analysis (D) of MSC-specific feline surface proteins CD44, CD73, CD90, and CD105 (green). Nuclei were visualized by staining with DAPI (blue)

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Stem Cell Research & Therapy

ISSN: 1757-6512