Table 1 Primer sequences used in this study

Feline pluripotency markers

OCT4

GGAGTCCCAGGACATCAAAG

GCCTGCACAAGTGTCTCTGC

224

SOX2

AGGATAAGTACACACTGCCCG

TGCATCATGCTGTAGCTGCC

180

NANOG

CTGGCCAAAGAATAACAACACTG

AGGTCTGGTTGCCCCATATT

138

c-Myc

GGACTGTCATCCTGCCCTAC

GGCATGAGCTCTTGGTAATGG

126

KLF4

GGGACTGTCATCCTGCCCTA

GGCATGAGCTCTTGGTAATGG

127

Human exogenous transgenes and Sendai virus (SV)

KOS

ATGCACCGCTACGACGTGAGCGC

ACCTTGACAATCCTGATGTGG

528

KLF4

TTCCTGCATGCCAGAGGAGCCC

AATGTATCGAAGGTGCTCAA

410

c-Myc

TAACTGACTAGCAGGCTTGTCG

TCCACATACAGTCCTGGATGATGATG

532

SV

GGATCACTAGGTGATATCGAGC

ACCAGACAAGAGTTTAAGAGATATGTATC

181

Human exogenous transgenes by Retro virus (RV)

OCT4

CCTCAAAGTAGACGGCATCGCA

TTATTCGGGGCACCTGCTTGA

251

SOX2

CCTCAAAGTAGACGGCATCGCA

AACCTGAGGCCCACAGTACGC

338

NANOG

ATGCCTCACACGGAGACTGT

AAGTGGGTTGTTTGCCTTTG

103

cMYC

CCTCGCAAGACTCCAGC

CAGAAGGTGATCCAGACTC

225

KLF4

CCCAATTACCCATCCTTCCT

ACGATCGTCTTCCCCTCTTT

127

Feline germ layer markers

GATA2

CTGCTGGTGAACGCCGA

GCGGTGGCAGCGTCC

140

GATA6

TCCTCCTCCTCTTCACCTCC

GTCAGTCAAGGCCATCCACG

150

AFP

CAGCCACTTGTTGCCAACTC

CACCGGGGTTTACAGGAGTC

117

ENOLASE

GGGACAATGACAAGACACGC

CACAACGCTCAGTTTCTTGC

107

NESTIN

AAACAGGGCCTACAGAG

ACAGGTGTCTCAAGGGTAG

293

CXCR4

GCTCAGGCGACTATGACTCC

TGCCCACTATGCCAGTCAAG

117

SMA

ACCCTTCAGCGTTCAGCTTC

TACAGAGCCCAGAACCGTTG

150

Feline mesenchymal stromal cell (MSC) markers

CD29

GGAAACTTGGTGGCATTGTT

GTTCCTTGTAAACGGGCTGA

180

CD34

CTTTAACTGTCACGGCGTTT

TGACTCGGGAACATTTGATT

198

CD44

TGGGTTGTTTGGCATCCAGTGC

CGTTTTCTTCAGTTGGTTCCCAGCC

100

CD73

AGCAAAGGGGCCACTAGCATCT

ACCCGAATGTCCCAGTGCAA

233

CD90

GAGCACACGTACCGCTCCCG

AGCAGCAGCAGCAGCATCCA

233

CD105

ATCACCTTTGGCGCCTTCCTTATC

GTGGTTGGTGCTACTGCTTTCTGA

144

Feline housekeeping gene

GAPDH

GCAAAGTGGACATTGTCGCC

CCTTCTCCATGGTGGTGAAG

203