Fig. 5
Bis-2-(5-phenyl acetamido-1,2,4-thiadiazol-2-yl) ethyl sulfide (BPTES) enhances the cytotoxic effect of tert-butyl hydroperoxide (TBHP) on bone mesenchymal stem cells (BMSCs). A total of 1 × 106 BMSCs were used in each group. BMSCs were incubated with PBS, TBHP (50 μM), BPTES (5 μM), or TBHP (50 μM) in the presence of BPTES (5 μM) for 24 h. A and B Cell proliferation was detected by CCK-8 (n = 5) and 5-ethynyl-2’-deoxyuridine (EdU) assay (n = 5). Representative EdU-positive cells (red) are shown. White Bars = 100 μm. C EdU-positive cells (red) were counted to calculate the percentage. D Lactate dehydrogenase (LDH) activity was measured by LDH kit (n = 5). E The ROS levels were measured by 2’−7’-dichlorofluorescein diacetate (1:1000, 20 μmol/L) (n = 5). White Bars = 100 μm. F Relative reactive oxygen species (ROS) intensity (green staining) was statistically analyzed. All data are representative of at least five separate experiments. All these experiments were repeated by at least five times. Analysis: ANOVA followed by a post hoc analysis (A, C, D, and F); **P < 0.01, ***P < 0.001