Table 2 Primary and secondary antibodies for Western Blot
Target | Antibody (Company, catalog number, usage) |
|---|---|
HSF1 | Abcam, Cambridge, UK, ab242138, 1:2000 |
Prominin-2 | Abcam, Cambridge, UK, ab74997, 1:600 |
BACH1 | Abcam, Cambridge, UK, ERP25428-72, 1:1000 |
Ubiquitin (NT) Rabbit Polyclonal Antibody | Beyotime, ShangHai, China, AG3164, 1:1000 |
GLS | Abcam, Cambridge, UK, ab202027, 1:1000 |
ACSL4 | Abcam, Cambridge, UK, ab155282, 1:10,000 |
PTGS2 | Bioss antibodies, Boston, Massachusetts, USA, bs-0732R, 1:1000 |
GPX4 | Abcam, Cambridge, UK, ab125066, 1:2000 |
Ferritin | Abcam, Cambridge, UK, ab183781, 1:1000 |
Histone-3 | Signalway antibody, College Park, Maryland, USA, HW029, 1:1000 |
β-Actin | Proteintech, Chicago, Illinois, USA, 20,536-1-AP, 1:5000 |
HRP conjugated goat anti-rabbit IgG (H + L) | Servicebio, WuHan, China, GB23303, 1:3000 |
Anti-EGFP rabbit pAb | Servicebio, WuHan, China, GB11602, 1:200 |
MMP-9 | Abcam, ab228402, 1:1000, Cambridge, UK |
ADAMTS5 | Invitrogen, PA5-32,142, 1:5000, Waltham, USA |
MMP-13 | Elabscience, E-AB-10064, 1:2000, WuHan, China |