Fig. 1

Characterization of iMSCs Morphology, Differentiation Potential, and Cell Surface Markers. A The left panel shows the typical colony morphology of iPSCs, the middle panel illustrates the morphological changes of iPSCs after 14 days of induced differentiation, and the right panel depicts the characteristic morphology of mesenchymal stem cells in P5 generation iMSCs. B Results of Alizarin Red S staining for calcium deposition, Oil Red O staining for lipid droplets, and Alcian Blue staining for cartilage matrix. C Flow cytometry analysis showing the expression of cell surface markers on iMSCs