Fig. 6
Effects of osteogenic induction medium containing conditioned medium (CM) from different macrophage groups on the osteogenic differentiation of PDLSCs. A ALP staining of PDLSCs on day 7. B Quantitative measurement of ALP activity in PDLSCs on day 7. C Alizarin Red staining of mineralized nodules formed by PDLSCs on day 21. D Semi-quantitative analysis of mineralized nodule formation in PDLSCs on day 21. E Relative expression levels of osteogenesis-related genes (ALP, OCN, RUNX2, and COL-I) on days 7 and 21. *P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant (P > 0.05)