Fig. 8
Immunofluorescence staining of macrophage markers and NF-κB signaling pathway in periodontal tissues. A Representative immunofluorescence images showing the expression of CD68, iNOS, and CD206 in periodontal tissues from Healthy, Periodontitis, and Periodontitis + iMSCs groups. B Quantitative analysis of the percentage of CD68 + , iNOS + , and CD206 + cells among total DAPI-stained nuclei. C Representative immunofluorescence images of p65 in the periodontal tissues across the three groups. D Quantitative analysis of the number of p65 nuclear-translocated cells in each field of view. E Representative immunohistochemical (IHC) images of IL-17 in periodontal tissues from different experimental groups. Scale bars: 100 μm (upper panels) and 50 μm (lower panels). F Quantitative analysis of the percentage area of IL-17-positive cells per field of view. *P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant (P > 0.05)