Fig. 1

Schematic Representation of f-hPL Production The leukoreduction filter is reversely perfused with saline to recover the residual contents within the filter. The collected contents are then subjected to centrifugation to obtain the f-pellet and supernatant. The supernatant is subsequently passed through an additional leukoreduction filter to achieve a sufficient protein concentration (27 mg/mL) for f-plasma. f-platelet and f-plasma are then combined, followed by three freeze-thaw cycles to facilitate platelet membrane disruption. After purification through centrifugation and filtration, the final f-hPL is collected