Table 2 Surface marker expression of MSCs grown in the medium supplemented with large scale production of f-hPL in quantum system
From: Human platelet lysate produced from leukoreduction filter contents enables sufficient MSC growth
| Â | Â | BM 1 | BM2 | BM3 |
|---|---|---|---|---|
f-hPL | PDGF-BB (ng/mL) | 12.4 | 17.7 | 10.5 |
Automated cell culture | Quantity of Bone marrow (mL) | 50 | 50 | 50 |
Age of donor (Y) | 23 | 25 | 22 | |
Total cell number | 4.6 × 107 | 12.6 × 107 | 8.4 × 107 | |
Viability | 91.8% | 95.3% | 89.4% | |
Cell size (um) | 16.5 | 21.5 | 15.6 | |
FACS | Isotype control | 2.0% | 1.7% | 0.9% |
CD45 | 3.7% | 1.9% | 2.3% | |
CD44 | 94.5% | 99.5% | 99.7% | |
CD90 | 95.3% | 99.5% | 99.5% | |
CD105 | 92.9% | 96.6% | 91.7% | |
CD166 | 88.8% | 93.0% | 82.1% |