Fig. 3

dECM@exo inhibits ICH-induced inflammation and improves MMP in astrocytes. (A) Morphological characteristics of primary astrocytes and their specific marker GFAP. (B) dECM@exo transfected into astrocytes. (C, D) Quantitative detection of the proportion of cells expressing Ki67(n = 3). (E) Cell viability of cells cultured with dECM or dECM@exo. (F) Cell viability of cells cultured with Hemin. (G-I) ELISA analysis of IL-1β, TNF-α, and IL-10 levels in astrocyte supernatants. (J) Western blot of TLR4 and NF-κB/P65, p-P65. (K) Statistical analysis of TLR4. (L) Statistical analysis of TLR4 mRNA. Scale bars: A and B, 50 μm; C, 100 μm. Data were expressed as mean ± standard deviation. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001